Melatonin-Sulfate Urine ELISA (6-Hydroxymelatonin Sulphate, 6-Sulfatoxymelatonin)

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Details for:  Melatonin-Sulfate Urine ELISA (6-Hydroxymelatonin Sulphate, 6-Sulfatoxymelatonin)

Enzyme immunoassay for the quantitative determination of Melatonin Sulfate (synonyms: 6-hydroxymelatonin sulfate, 6-sulfatoxymelatonin) in human urine. For research use only. Not for use in clinical diagnostic procedures.

Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed colour is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.

The pineal gland (corpus pineale) has been called a neuroendocrine transducer because of its important role in photoperiodism. The major hormone of the pineal gland is N-acetyl-5-methoxy-tryptamine or melatonin which is synthezised from the amino acid tryptophane. Melatonin has its highest levels in plasma during nighttime. Its characteristic nocturnal surge appears to encode temporal information such as length of night. Regulation of the melatonin secretion is under neural control. Sympathetic innervation seems to play a major role via its release of noradrenaline. Most of the circulating melatonin is metabolized in the liver to 6-hydroxymelatonin and subsequently to 6 sulfatoxymelatonin which is excreted into the urine.

The concentration of 6-hydroxymelatonin sulfate in urine correlates well with the total level of melatonin in the blood during the collection period.c procedures.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE54031

Melatonin-Sulfate Urine ELISA (6-Hydroxymelatonin Sulphate, 6-Sulfatoxymelatonin)
List Price $577
Kit size 12 x 8
Method ELISA
Incubation time 1 x 2 h, 1 x 30 min
Standard range 1.7 - 420 ng/mL
Specimen / Volumes 10 µL urine
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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