Histamine ELISA

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Details for:  Histamine ELISA

Enzyme immunoassay for the quantitative determination of histamine in human plasma, urine, EDTA whole blood and cell culture supernatants. For research use only. Not for use in clinical diagnostic procedures. Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed colour is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.

In humans, histamine (ß-imidazolethylamine) is the most important mediator and is mostly found in the initial phase of an anaphylactic reaction ("immediate type" allergy). Histamine is derived by the enzymatic decarboxylation of histidine. In the organism, histamine is present in nearly all tissues, and it is mainly stored in the metachromatic granula of mast cells and the basophilic leukocytes. It is present in an inactive bound form and is only released as required. Like several other mediators, histamine does not only mediate various clinical symptoms of anaphylaxis but also induces a series of effects which are directed towards a termination of the anaphylactic reaction. The biological action of histamine in tissue is guaranteed by three different surface receptors, i.e. H1, H2 and H3 receptors.
IBL offers additional reagents for the performance of a Histamine Release test in heparinized whole blood (REF RE95000).

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE59221

Histamine ELISA
List Price $366
Kit size 12 x 8
Method ELISA
Incubation time 1 x 3 h, 1 x 40 min
Standard range 0.35 - 150 ng/mL
Specimen / Volumes 100 µL plasma, 50 µL urine
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only