Fully automated processing of dementia markers using TECAN Freedom EVOlyzer®

Products
hTAU total ELISA*
*In USA for research use only.
Not for use in clinical diagnostic procedures.

Manual processing of immunoassays by IBL International for determining amyloid-beta (1-42), amyloid-beta (1-40) and hTAU total in CSF generates very high-quality results.

Nevertheless, the need for an automated processing of dementia markers increases with increasing sample throughput. Therefore, we have verified the immunoassays Amyloid-beta (1-42) CSF ELISA, Amyloid-beta (1-40) CSF ELISA and hTAU total ELISA1 for the TECAN Freedom EVOlyzer®.

 

Inter-assay (table 1) and intra-assay variance (table 2) in fully automated processing

  Amyloid-β (1-40)
CSF ELISA
Amyloid-β (1-42)
CSF ELISA
hTAU total ELISA
Sample Mean [pg/mL] CV [%] Mean [pg/mL] CV [%] Mean [pg/mL] CV [%]
1 2,814 5.7 184 4.3 691 4.2
2 19,880 4.9 611 2.7 959 3.0
3 8,900 1.9 306 1.9 760 2.2
4 11,672 3.6 511 2.7 769 2.9
5 6,950 5.0 774 5.0 359 5.6
6 13,765 4.9 666 4.0 2,367 4.0
Table 1: Inter-Assay Variance

 

  Amyloid-β (1-40) CSF ELISA Amyloid-β (1-42) CSF ELISA hTAU total ELISA
Sample Mean [pg/mL] CV [%] Mean [pg/mL] CV [%] Mean [pg/mL] CV [%]
1 5,740 2.4 357 3.6 2,224 1.7
2 8,290 3.5 705 4.4 871 1.7
3 11,199 7.5 1,039 6.1 1,348 2.8
Table 2: Intra-Assay Variance

The results of the manual and automated processing correlate perfectly


Image 1: Correlation Amyloid-beta (1-40) Manual-EVOlyzer®

Image 2: Correlation Amyloid-beta (1-42) Manual-EVOlyzer®

 

The data show that TECAN Freedom EVOlyzer® processes the immunoassays in excellent quality and reproducibility.
The combined use of assays, process script and the TECAN Freedom EVOlyzer® has to be validated individually on site by each laboratory.