Cortisol ELISA

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Details for:  Cortisol ELISA
Enzyme immunoassay for the quantitative determination of Cortisol in human serum and plasma.

Cortisol (hydrocortisone, compound F) is the main glucocorticoid in humans and is produced in the zona fasciculata of the adrenal cortex. 90 % of the circulating cortisol are bound to corticoid binding globulin (CBG, Transcortin), ca. 7 % are bound to albumin and only 1–3 % are unbound. Only the latter part represents the active form of cortisol.
In humans there is a physiological fluctuation of cortisol achieving the highest level in the morning and the lowest during midnight. This fluctuation of cortisol plasma level is reflected in saliva normally with a peak in the first 90 minutes after wake up.
Because of the diurnal fluctuation of cortisol levels it is necessary to take several samples for an individual cortisol profile or during dynamic tests like dexamethasone-suppression- or ACTH-stimulation-test.

This kit is available for research use only in the USA.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE52061

Cortisol ELISA
List Price $180
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1 h, 1 x 15 min
Standard range 20 - 800 ng/mL
Specimen / Volumes 20 µL serum, plasma
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only