Cortisol Saliva ELISA

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Details for:  Cortisol Saliva ELISA

Sensitive and reliable

Free cortisol measurement in saliva is a convenient way of assessing adrenal gland function. Sustained abnormally high or low cortisol levels are often found in people with adrenal function imbalance. Adrenal dysfunction may occure in many related clinical conditions, which include fibromyalgia, hypo-thyroidism, chronic fatigue syndrome and premature menopause.

The Cortisol Saliva ELISA is recommended for patients with e.g. reduced tolerance for stress, fatigue, sleep disturbances and depression.

Advantages of the IBL International Cortisol Saliva ELISA

  • Excellent performance due to enzymatic signal amplification
  • Easy to adapt to open automated instruments
  • EU: CE, US: IVD
  • Suitable for measurement of total cortisol in saliva and diluted serum

Assay characteristics

Analytical sensitivity: 0.05 ng/mL
Functional sensitivity (20 % CV): 0.3 ng/mL
Analytical specificity
Cortisone4.2 %
Prednisone2.5 %
 Corticosterone1.4 %
 Desoxycorticosterone0.9 %
 17-OH-Progesterone0.4 %
Intra-assay CV:3,1-7,3 %at 2,7-23,4 ng/mL 
Inter-assay CV:6,4-9,3 %at 5,4-23,5 ng/mL 

Excellent correlation with the Cortisol Luminescence IA

The cortisol content of 454 saliva samples was assessed both by the IBL Cortisol ELISA and the IBL Cortisol Luminescence Immunoassay (LUM IA). All samples were stored frozen at -20 °C and centrifuged at 3,000 g before use.

The results of the method comparison study show very good correlation between the Cortisol ELISA and the Cortisol LUM IA.

Excerpt from the Instructions for Use

Enzyme immunoassay for the in-vitro diagnostic quantitative determination of free Cortisol in human saliva and of total Cortisol in diluted serum as an aid in the assessment of Cushing Syndrome and Addison’s Disease.Cortisol is a corticosteroid or glucocorticoid produced by the adrenal cortex. It is usually referred to as the "stress hormone" as it is involved in response to stress and anxiety. It increases blood pressure and blood sugar, and reduces immune responses. Free cortisol monitoring is indicated in diseases with abnormal glucocorticoid production such as Cushing`s syndrome and Addison’s disease. Because of the diurnal fluctuation of cortisol levels, it is necessary to take several samples for an individual cortisol profile or during dynamic tests like the dexamethasone-suppression or ACTH stimulation (Westerman, Clin. Lab. (2004) 50; 11-24). Furthermore adrenal dysfunction can be pointed out through concomitant measurement of the most important stress hormones cortisol and DHEA. Cortisol helps evaluate the stress response, whereas DHEA helps determine the stress adaptation. Adrenal imbalance may be a factor in many related clinical conditions that include e.g. fibromyalgia, hypothyroidism, chronic fatigue syndrome and depression. Our Cortisol saliva is very well published, please do not hesitate to contact us for further information.

NEW: Cortisol can also be found in hair for long stress studies e.g.. Our luminescence assay has already been tested in such samples, more information are available upon request!

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE52611

Cortisol Saliva ELISA
List Price $175
Kit size 12 x 8
Method ELISA
Incubation time 1 x 2 h, 1 x 30 min
Standard range 0.3 - 40 ng/mL
Specimen / Volumes 50 µL saliva/without extraction, serum after dilution
Substrate / isotope TMB 450 nm
Regulatory Status: IVD