GRO (Growth Related Oncogene) belonging to IL-8 family is a polypeptide which has three isoforms (α, β and γ), and it inhibits proliferation of endothelial cells. GRO/CINC-1 (cytokine-induced neutrophil chemo attractant 1) was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells (NRK-52E). CINC-1 is a member of the alpha (CXC) subfamily of chemokines. Three additional rat CXC chemokines (CINC-2α, CINC-2β, CINC-3/MIP-2) have been identified. The protein sequence of CINC-1 is 63 - 67% identical to that of CINC-2α, CINC-2β and CINC-3/MIP-2. In addition, each of GROα, GROβ and GROγ is sharing 68%, 71% and 69% identity with CINC-1. This has been suggested that CINCs are the rat counterparts of human GROs. GROα/MGSA has a high homology with IL-8 in amino acid sequences.
| Catalog number | JP27162 |
| Kit size | 12 x 8 |
| Method / Technology | ELISA |
| Incubation time | 1 x 1 h, 2 x 30 min |
| Standard range | 4.7 - 300 pg/mL |
| Specimen / Volumes | 100 µL serum, EDTA plasma, cell culture supernatant |
| Substrate / isotope | TMB 450 nm |