5-alpha Dihydrotestosterone (DHT) ELISA

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Details for:  5-alpha Dihydrotestosterone (DHT) ELISA

Enzyme immunoassay for the quantitative determination of 5α-Dihydrotestosterone in human serum. For research use only. Not for use in diagnostic procedures.

5α-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a class called androgens. DHT is a C19 steroid.

Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed colour is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. DB52021

5-alpha Dihydrotestosterone (DHT) ELISA
List Price $218
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1 h, 1 x 10 min
Standard range 25 - 2500 pg/mL
Specimen / Volumes 50 µL serum/without extraction
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only