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Details for:  ANA-HEp2 Screen ELISA

ANA HEp-2 Screen ELISA is a solid phase enzyme immunoassay for the combined qualitative detection of IgG antibodies against HEp2 cells in human serum. Each well is coated with lysed HEp2 cells. The test collectively detects, in one well, total ANAs against double stranded DNA (dsDNA), histones, SS-A (Ro), SS-B (La), Sm, snRNP/Sm, Scl-70, PM-Scl, Jo-1 and centromeric antigens along with sera positive for HEp2 immunofluorescence test (IFT). For research use only. Not for use in diagnostic procedures.

Test principle

Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Sample antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE70151

List Price $268
Kit size 12 x 8
Method ELISA
Incubation time 3 x 30 min
Standard range cut-off control
Specimen / Volumes 10 µL serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only