dsDNA Screen ELISA

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Details for:  dsDNA Screen ELISA

dsDNA Screen ELISA is a solid phase enzyme immunoassay with human recombinant double-stranded DNA (dsDNA) for the combined quantitative and qualitative detection of IgG, IgA and IgM antibodies against dsDNA in human serum. Anti-dsDNA antibodies mainly recognize the phosphate units of the DNA, thus these autoantibodies also bind single stranded DNA (ssDNA). To ensure correct quantitation of anti-dsDNA antibodies the used antigen has been proven to be free of contamination with ssDNA. For research use only. Not for use in diagnostic procedures.

Test principle

Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Sample antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE70191

dsDNA Screen ELISA
List Price $261
Kit size 12 x 8
Method ELISA
Incubation time 3 x 30 min
Standard range 0 - 300 U/mL, cut-off 20 U/mL
Specimen / Volumes 10 µL serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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