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 Histone-H1-Ab ELISA

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Details for:  Histone-H1-Ab ELISA

Histone-H1-Ab ELISA is a solid phase enzyme immunoassay with human native histone H1 isolated from the eukaryotic celline HeLa for the quantitative and qualitative detection of antibodies against histone H1 in human serum. For research use only. Not for use in diagnostic procedures.

Test principle

Histones are small basic proteins of 11 to 21 kDa constituting a set of interacting proteins that organize and constrain the topology of DNA in most eukaryotic cells into a particle called nucleosome. In this particle the four histones H2A, H2B, H3 and H4 form an octamer (H2A, H2B, H3, H4)2 around which 146 bp of dsDNA is wrapped by 1 ¾ turns. Histone H1 interacts with the nucleosome and together with linked-DNA connects neighboring nucleosomes. Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Sample´s antibodies, if presentin the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samp-les in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The rate of color for-mation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE70281

Histone-H1-Ab ELISA
List Price $372
Kit size 12 x 8
Method ELISA
Incubation time 3 x 30 min
Standard range 0 - 300 U/mL, cut-off 15 U/mL
Specimen / Volumes 10 µL serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only