Rheumatoid Factor IgA/IgG/IgM ELISA is a solid phase enzyme immunoassay with highly purified Fc fragment of human immunoglobuline (IgG) for the separate quantitative and qualitative detection of IgG, IgM and IgA rheumatoid factors (RF) in human serum. For research use only. Not for use in diagnostic procedures
Test principle
Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.
For concrete data please consult the Instruction for Use in the download box on the right side.
