Acetylcholine Receptor-Ab (ARAb) Blocking RIA

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Details for:  Acetylcholine Receptor-Ab (ARAb) Blocking RIA

Radio immunoassay for the quantitative determination of blocking autoantibodies against the acetylcholine receptor in human serum. For research use only. Not for use in clinical diagnostic procedure.

The samples are incubated with labeled 125I–alpha-bungarotoxin acetylcholine receptors. The blocking acetylcholine receptor antibodies contained in the samples compete with the 125I–alpha-bungarotoxin for a limited number of binding sites on the receptors. If samples are positive, blocking antibodies bind to the acetylcholine receptors. The resulting complexes of acetylcholine receptors and specific antibodies are precipitated with Con-A Sepharose. After centrifugation and aspiration of the supernatant the precipitates are counted for 125I by use of a gamma counter. The measured counts are inversely proportional to the concentration of blocking acetylcholine receptor autoantibodies present in the sample. The concentration is expressed as the activity of inhibition (%) of the binding of 125I-alpha-bungarotoxin to the receptors.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE21031

Acetylcholine Receptor-Ab (ARAb) Blocking RIA
List Price $929
Kit size 100
Method RIA
Incubation time 1 x 3h, 1 x 1 h
Standard range negative and positive control
Specimen / Volumes 20 µL serum
Substrate / isotope 125I < 150 kBq
Regulatory Status: For research use only

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