Thyroglobulin (hTG) ELISA

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Details for:  Thyroglobulin (hTG) ELISA

Thyroglobulin ELISA is an indirect solid phase enzyme immunoassay for the quantitative detection of thyroglobulin (Tg) in human serum. The assay employs selected specific monoclonal antibodies against human thyroglobulin (Tg). For research use only. Not for use in diagnostic procedures.

Test principle

Undiluted serum samples are incubated in the microplates coated with monoclonal antibodies against human thyroglobulin (Tg). Tg, if present in the specimen, bind to the antibodies. The unbound fraction is washed off in the following step. Afterwards monoclonal anti-Tg immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMBsubstrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The rate of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of Tg in the sample.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE70971

Thyroglobulin (hTG) ELISA
List Price $350
Kit size 12 x 8
Method ELISA
Incubation time 3 x 1 h
Standard range 0 - 50 ng/mL, recovery 10 ng/mL
Specimen / Volumes 100 µL serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only