Enzyme immunoassay for the quantitative determination of DHEA-S in human saliva. For research use only. Not for use in diagnostic procedures.
Test principle
DHEA-S (antigen) in the sample competes with horseradish peroxidase DHEA-S (enzyme-labelled antigen) for binding onto the limited number of anti DHEA-S (antibody) sites on the microplates (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. The enzyme substrate (H2O2) and the TMB-Substrate (TMB) are added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the OD are determined. DHEA-S concentration in the sample is calculated based on a series of standard. The colour intensity is inversely proportional to the DHEA-S concentration of in the sample.
For concrete data please consult the Instruction for Use in the download box on the right side.
