Melatonin direct Serum/Plasma/Saliva RIA (Non-Extraction)

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Details for:  Melatonin direct Serum/Plasma/Saliva RIA (Non-Extraction)

Radio immunoassay for the quantitative determination of melatonin in human serum, plasma and saliva. For research use only. Not for use in clinical diagnostic procedures.

Radio immunoassay (RIA) based on the competition principle. A limited amount of specific antibody (Ab) reacts with the corresponding antigen (*Ag) labelled with the 125I-radioisotope. Upon addition of an increasing amount of the Ag (sample), a correspondingly decreasing fraction of *Ag added is bound to the antibody. After separation of the bound from the free *Ag by precipitation and centrifugation, the amount of bound radioactivity of the precipitates is measured in a Gamma counter. Results of samples can be determined directly using the standard curve. The pineal gland (corpus pineale) has been called a neuroendocrine transducer because of its important role in photoperiodism. The major hormone of the pineal gland is N-acetyl-5-methoxy-tryptamine or melatonin which is synthezised from the amino acid tryptophane. Melatonin has its highest levels in plasma during nighttime. Its characteristic nocturnal surge appears to encode temporal information such as length of night. Regulation of the melatonin secretion is under neural control. Sympathetic innervation seems to play a major role via its release of noradrenaline. Most of the circulating melatonin is metabolized in the liver to 6-hydroxymelatonin and subsequently to 6-sulfatoxymelatonin which is excreted into the urine.The concentration of 6-hydroxymelatonin sulfate in urine correlates well with the total level of melatonin in the blood during the collection period.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE29301

Melatonin direct Serum/Plasma/Saliva RIA (Non-Extraction)
List Price $375
Kit size 100
Method RIA (DAb)
Incubation time 1 x 2 h, 1 x 18 h, 1 x 15 min (1 x 18 h, 1 x 15 min)
Standard range 3 - 750 pg/mL (3 - 300 pg/mL)
Specimen / Volumes 200 µL serum, plasma (500 µL saliva)
Substrate / isotope 125I < 200 kBq
Regulatory Status: For research use only