Estriol Saliva ELISA

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Details for:  Estriol Saliva ELISA

Enzyme immunoassay for the quantitative determination of Estriol in human saliva. For research use only. Not for use in diagnostic procedures.

Test principle

Estriol (antigen) in the sample competes with horseradish peroxidase estriol (enzyme-labelled antigen) for binding onto the limited number of antiestriol (antibody) sites on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The colour intensity is inversely proportional to the Estriol concentration in the sample. Estriol concentration in the sample is calculated through a calibration curve.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE52291

Estriol Saliva ELISA
List Price $281
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1h, 1 x 15 min
Standard range 2.5 - 4000 pg/mL
Specimen / Volumes 50 µL Saliva/without extraction
Substrate / isotope TMB, 450 nm
Regulatory Status: For research use only