Cortisol Saliva ELISA

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Details for:  Cortisol Saliva ELISA

Sensitive and reliable

Free cortisol measurement in saliva is a convenient way of assessing adrenal gland function. Sustained abnormally high or low cortisol levels are often found in people with adrenal function imbalance. Adrenal dysfunction may occure in many related conditions, which include fibromyalgia, hypo-thyroidism, chronic fatigue syndrome and premature menopause.

The Cortisol Saliva ELISA is widely used in research studies connected to the reduced tolerance for stress, fatigue, sleep disturbances and depression.

Advantages of the IBL International Cortisol Saliva ELISA

  • Excellent performance due to enzymatic signal amplification
  • Easy to adapt to open automated instruments
  • Suitable for measurement of total cortisol in saliva and diluted serum

Assay characteristics

Analytical sensitivity: 0.05 ng/mL
Functional sensitivity (20 % CV): 0.3 ng/mL
Analytical specificity
(cross-reactivity):
Cortisone4.2 %
Prednisone2.5 %
 Corticosterone1.4 %
 Desoxycorticosterone0.9 %
 17-OH-Progesterone0.4 %
Intra-assay CV:3,1-7,3 %at 2,7-23,4 ng/mL 
Inter-assay CV:6,4-9,3 %at 5,4-23,5 ng/mL 

Excellent correlation with the Cortisol Luminescence IA

The cortisol content of 454 saliva samples was assessed both by the IBL Cortisol ELISA and the IBL Cortisol Luminescence Immunoassay (LUM IA). All samples were stored frozen at -20 °C and centrifuged at 3,000 g before use.

The results of the method comparison study show very good correlation between the Cortisol ELISA and the Cortisol LUM IA.

Excerpt from the Instructions for Use


Enzyme immunoassay for the quantitative determination of free Cortisol in human saliva. For research use only. Not for use in diagnostic procedures.
Cortisol (hydrocortisone) is the main glucocorticoid in humans and is produced in the zona fasciculata of the adrenal cortex from low-density lipoprotein cholesterol by several enzymatic reactions. 90 % of the circulating cortisol are bound to corticoid binding globulin (CBG, Transcortin), ca. 7 % are bound to albumin and only 1–3 % are unbound. Only the latter part represents the active form of cortisol. During passive diffusion into the salivary glands, approximately one-third of the free cortisol is lost through conversion to cortisone. In saliva there is a diurnal fluctuation of the cortisol concentration in adult humans, reaching a peak around 30 to 60 minutes after awakening, so called Cortisol awakening response, and falling to lower levels in the afternoon which remain through the next morning.
Our Cortisol saliva is very well published, please do not hesitate to contact us for further information.

NEW: Cortisol can also be found in hair for long stress studies e.g.. Our luminescence assay has already been tested in such samples, more information are available upon request!

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. 30172091

Cortisol Saliva ELISA
List Price $189
Kit size 12 x 8
Method ELISA
Incubation time 1 x 2 h, 1 x 30 min
Standard range 0.3 - 40 ng/mL
Specimen / Volumes 50 µL saliva
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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