Aflatoxin M1 ELISA

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Details for:  Aflatoxin M1 ELISA
Enzyme immunoassay for the quantitative determination of Aflatoxin M1 in milk and milk products.

The IBL International Aflatoxin M1 quantitative test is based on the principle of the enzyme linked immunosorbent assay. An aflatoxin conjugate is bound on the surface of a microtiter plate. Aflatoxin M1 containing samples or standards and an antibody directed against aflatoxin M1 are given into the wells of the microtiter plate. Immobilized and free aflatoxin M1 compete for the antibody binding sites. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugate against the antibody is given into the wells and after another hour incubation, the plate is washed again. Then a substrate solution is added and incubated for 20 minutes,
resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of aflatoxin M1 is indirectly proportional to the colour intensity of the test sample.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE59581

Aflatoxin M1 ELISA
List Price $478
Kit size 12 x 8
Method ELISA
Incubation time 2 x 1h, 1 x 20 min.
Standard range 10 - 1000 pg/ml
Specimen / Volumes 100µl milk or prepared milk products
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only