Caspase-9 ELISA

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Details for:  Caspase-9 ELISA
Caspases are the executioners of apoptosis. These cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis. Caspase-9, formerly called Apaf-3, binds to Apaf-1 in the presence of cytochrome c and dATP (3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3; caspase- 9 thus is the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Caspase-9 plays a crucial role in all pathologies involving apoptotic processes. Specifically the involvement of caspase-9 has been described in human gastric cancer, ovarian carcinoma, neuroblastoma, gliomas as well as in Alzheimer´s disease, Huntington´s disease and osteoarthritis.

Synonyms: cysteinyl-aspartate specific protease-9, Apaf-3
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. BE52091

Caspase-9 ELISA
List Price $501
Kit size 12 x 8
Method ELISA
Incubation time 1 x 2 h, 1 x 1h, 1 x 10min
Standard range 1.6 - 100 ng/mL
Specimen / Volumes 50µL serum, plasma, cell culture supernatants et al.
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only