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 Total IgE ELISA

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Details for:  Total IgE ELISA
The Total-IgE ELISA Test Kit has been designed for the the detection and the quantitative determination of total IgE antibodies in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of IBL International. This assay is intended research Use only. It is not intended for use in diagnostic procedures.

PRINCIPLE OF THE TEST
The Total-IgE ELISA is based on the principle of the enzyme immunoassay (EIA). A monoclonal mouseanti-human IgE antibody is bound on the surface of the microtiter strips. Undiluted patient serum or readyto-use standards are pipetted into the wells of the microtiter plate together with anti-human-IgE-peroxidase conjugate. A sandwich complex between the serum IgE and the two antibodies develops. After a 30 minutes´ incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then the substrate (TMB) solution is pipetted and incubated for 15 minutes, inducing the development of a blue dye in the wells. The colour development is terminated by the addition of a stop solution, which changes the colour from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgE antibodies is directly proportional to the intensity of the colour.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE59061

Total IgE ELISA
List Price $186
Kit size 12 x 8
Method ELISA
Incubation time 1 x 30 min, 1x 15 min
Standard range 5 - 1000 IU/mL
Specimen / Volumes 10 µL serum, plasma
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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