BoxB from HMGB1, human & mouse, with tag, LPS-free - 15µg

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Details for:  BoxB from HMGB1, human & mouse, with tag, LPS-free - 15µg
Manufacturer product code: HM-053

BoxB is one of the DNA binding domains of HMGB1 protein (HMG boxes). Structure-function analysis revealed that the cytokine activity of HMGB1 can be reproduced by recombinant BoxB. It recapitulates the cytokine activity of full length HMGB1 and efficiently activates macrophages to release tumor necrosis factor (TNF) and other proinflammatory cytokines (Li et al. Structural basis for the proinflammatory cytokine activity of high mobility group box 1. Mol Med 2004, 9:37-45).

BoxB from HMGB1 is produced in E.coli (Müller et al. Thermodynamics of HMGB1 interaction with duplex DNA. Biochemistry 2001, 40: 10254-61) from an expression plasmid coding for the mammalian sequence, which is totally identical in human and mouse, preceded by four amino acids (MARI) that have been added for the purpose of expression in bacteria (Bianchi et al. The DNA binding site of HMG1 protein is composed of two similar segments (HMG boxes), both of which have counterparts in other eukaryotic regulatory proteins. EMBO J 1992, 11: 1055-63).
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Catalog No. 30141642

BoxB from HMGB1, human & mouse, with tag, LPS-free - 15µg
List Price $180
Type recombinant protein
Available Sizes 15 µg
Regulatory Status: For research use only