ACTH ELISA

Enzyme immunoassay use in the quantitative determination of human adrenocorticotropic hormone (ACTH) in EDTA-plasma. For research use only. Not for use in clinical diagnostic procedures.

This ELISA kit is designed, developed and produced for the quantitative measurement of human ACTH in EDTA-plasma sample. The assay utilizes the two-site “sandwich” technique with selected antibodies that bind to N-terminal and C-terminal epitopes of ACTH. Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to the C-terminal of human ACTH. Immediately, a horseradish peroxidase (HRP) conjugated anti N-terminal of human ACTH antibody is added to each well. After the first incubation period, a “sandwich” of solid-phase polyclonal antibody - human ACTH – HRP conjugated monoclonal antibody” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (i.e. ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human ACTH in the test sample. A standard curve is generated by plotting the absorbance versus the respective human ACTH concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human ACTH in test samples is determined directly from this standard curve.

IBL presents a new, simplified adrenocorticotropic hormone (ACTH) ELISA, with the following advantages:

• Assay time of 2 hours
• Easy calculation of results (single plate reading at 450 nm)
• Wide dynamic range (0 - 416 ng/mL)

Synonyms: Adrenocorticotropic hormone, Corticotropin, Cosyntropin