For the direct quantitative determination of 5α-Androstane-3α,17β-diol Glucuronide (3αDiol G) by enzyme immunoassay in human serum. For research use only. Not for use in clinical diagnostic procedures.
5α-Androstane-3α, 17β-diol glucuronide is a C19 steroid and is either abbreviated as 3α Diol G, 5α Diol G or simply, α Diol G. It is produced mainly as a metabolite of testosterone and dihydrotestosterone (DHT). It is largely produced in target peripheral tissues such as the skin, especially around hair follicles.
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed colour is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.
For concrete data please consult the Instruction for Use in the download box on the right side.
