Details for:
Angiotensin I (PRA) RIA (CT)
Radioimmunoassay of Angiotensin I for the determination of Plasma Renin Activity (PRA) in human plasma. For research use only.
PRINCIPLE OF THE ASSAY
The Angiotensin I RIA serves for the quantitative determination of plasma renin activity (PRA) by the radioimmunoassay of the product of the reaction, angiotensin I.
The generation of angiotensin I is the result of the enzymatic cleavage of the renin substrate, angiotensinogen, in plasma samples in the presence of ACE inhibitor (ACE - Angiotensin-Converting Enzyme), an enzymatic inhibitor that blocks the conversion of angiotensin I to angiotensin II. The immunoassay of angiotensin I is a radioimmunological competition assay. Unknown samples, control, and calibrators are incubated in polyclonal antibody-coated tubes with 125I-labeled angiotensin I as tracer. After incubation, the contents of the tubes are aspirated. The bound radioactivity is then determined in a gamma counter. A standard curve is established and unknown values are determined by interpolation from the standard curve.
For concrete data please consult the Instruction for Use in the download box on the right side.
