25-OH-Vitamin D ELISA

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Details for:  25-OH-Vitamin D ELISA

Vitamin D refers to a group of fat-soluble secosteroids being responsible for intestinal absorption of calcium, iron, magnesium, phosphate and zinc. The most important vitamin Ds in humans are vitamin D3 (cholecalciferol) and vitamin D2 (ergocalciferol). Vitamin D3 is synthesized in the skin from the cholesterol precursor 7-dehydrocholesterol and is the major source of vitamin D in humans. In contrast, vitamin D2 is mainly a synthetic product, which is predominantly present as a food additive in certain foods such as milk. In the liver vitamin D is further metabolized via hydroxylation to 25-hydroxyvitamin D (25(OH)-vitamin D). 25(OH)-vitamin D is the major circulating metabolite of vitamin D. 25(OH)-vitamin D is the precursor for other vitamin D metabolites, but shows also limited activity by itself. The most active metabolite is 1,25-dihydroxyvitamin D, which is produced in the kidney by 1-hydroxylation of 25(OH) vitamin D.


Advantages of our assay are:



  • Direct assay without extraction

  • Ready-to-use reagents

  • Easily adaptable on open ELISA automates

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE53041

25-OH-Vitamin D ELISA
List Price $376
Kit size 12 x 8
Method ELISA
Incubation time 1 x 90min., 1 x 30min., 1 x 15min.
Standard range 0 - 130 ng/mL
Specimen / Volumes 25µL serum
Substrate / isotope TMB 450nm
Regulatory Status: For research use only

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