Pregnenolone ELISA

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Details for:  Pregnenolone ELISA

Enzyme immunoassay for the direct quantitative determination of Pregnenolone in human serum.

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario.Competition occurs between an unlabeled antigen (present in standards, control and samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of pregnenolone in the sample. A set of standards is used to plot a standard curve from which the amount of pregnenolone in samples and controls can be directly read.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. DB52031

Pregnenolone ELISA
List Price $266
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1 h, 1 x 10 min
Standard range 0.1 - 25.6 ng/mL
Specimen / Volumes 50 µL serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only