Homocysteine ELISA

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Details for:  Homocysteine ELISA
The Axis® Homocysteine Enzyme Immunoassay is intended for the quantitative determination of total homocysteine in plasma or serum.

Homocysteine is a thiol-containing amino acid produced by the intracellular demethylation of methionine. Homocysteine is metabolised to either cysteine or to methionine. In the vitamin B6 dependent trans-sulphuration pathway homocysteine is irreversibly catabolised to cysteine. A major part of homocysteine is remethylated to methionine, mainly by the folate and cobalamindependent enzyme methionine synthase. Homocysteine accumulates in the cell and is exported to the circulation when these reactions are impaired 1-4. Homocysteine circulates in plasma mostly in its oxidised form bound to proteins and is measured as total homocysteine, (tHcy), the sum of free and protein bound. Severely elevated concentrations of tHcy are found in subjects with homocystinuria, a rare genetic disorder of the enzymes involved in the metabolism of homocysteine. Patients with homocystinuria exhibit mental retardation, early arteriosclerosis and arterial and venous thromboembolism. Other less severe genetic defects which lead to moderately elevated levels of homocysteine are also found. Epidemiological research has shown that different patient groups might have elevated homocysteine levels in blood. More than 40 epidemiological studies have shown that elevated tHcy is an independent risk factor of cardiovascular disease (CVD). A metaanalysis of 27 of these studies, including more than 4000 patients, estimated that a 5 µmol/L increase in tHcy was associated with an odds ratio of 1.7 for coronary artery disease, 1.5 for cerebrovascular disease or the same increase in risk as for 0.5 mmol/L increase in cholesterol9. Peripheral arterial disease also showed a strong association. Patients with chronic renal disease experiences an excess morbidity and mortality due to arteriosclerotic CVD. Elevated concentration of tHcy is a frequent observed finding in the blood of these patients. Although they may lack some of the vitamins involved in the metabolism of homocysteine, the increased levels of tHcy are mainly due to impaired removal of homocysteine from the blood by the kidney.

Distributed by IBL International, Germany. The instructions for use can be sent upon request.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. AX51301

Homocysteine ELISA
List Price $525
Kit size 12 x 8
Method ELISA
Incubation time 2 x 30 min, 1 x 20 min
Standard range 2 - 50 µmol/L
Specimen / Volumes 25 µL plasma
Substrate / isotope TMB 450 nm
Regulatory Status: IVD

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