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 C-Peptide ELISA

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Details for:  C-Peptide ELISA

The IBL C-Peptide ELISA is an enzyme immunoassay for the quantitative measurement of C-Peptide in serum, plasma and urine. For research use only. Not for use in diagnostic procedures.

Test principle

The IBL C-Peptide ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with anti-mouse antibodies, which binds a monoclonal antibody directed towards a unique antigenic site on the C-Peptide molecule. Endogenous C-Peptide of a sample competes with a C-Peptide-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of C-Peptide in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of C-Peptide in the sample.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE53011

C-Peptide ELISA
List Price $286
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1h, 1 x 30 min, 1 x 20min
Standard range 0.2 - 16 ng/mL
Specimen / Volumes 100 µL serum, plasma
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only