Insulin IRMA (CT)

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Details for:  Insulin IRMA (CT)

Immunoradiometric Assay (Coated tube) for the quantitative determination of human Insulin in serum. For research use only. Not for use in diagnostic procedures.

Test principle

The Insulin IRMA (CT) is an immunoradiometric assay based on coated-tube separation. Mabs1, the capture antibodies, are attached to the lower and inner surface of the plastic tube. Calibrators or samples added to the tubes will at first show low affinity for Mabs1. Addition of Mab2, the signal antibody labelled with 125I, will complete the system and trigger the immunological reaction. After washing, the remaining radioactivity bound to the tube reflects the antigen concentration. The use of several distinct Mabs avoids hyperspecificity, common to two-site IRMA, as well as a need of a shaker or incubation at 37°C.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. MG13021

Insulin IRMA (CT)
List Price $167
Kit size 96
Method IRMA (CT)
Incubation time 1 x 2 h
Standard range 5.7 - 440 µIU/mL
Specimen / Volumes 50 µL serum, plasma
Substrate / isotope 125I < 350 kBq
Regulatory Status: For research use only