ACTH Radioimmunoassay

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Details for:  ACTH Radioimmunoassay

The IBL-International ACTH IRMA (CT) kit has been designed for the quantitative determination of human adrenocorticotropic hormone levels in human plasma. For research use only. Not for use in diagnostic procedures

Test principle

The IBL-International ACTH IRMA (CT) kit is an immunoradiometric (IRMA) assay utilizing polyclonal antibodies directed against ACTH 1-16 and ACTH 24-39. The use of these antibodies guarantees that only ACTH is detected. The anti-ACTH 24-39 (C-terminal fragment) is labeled with 125I. The antibody directed against ACTH 1-16 (N-terminal fragment) is coated onto the tubes. ACTH in samples is bound both to the tubes and to the 125I-anti ACTH (24-39) antibody. After incubation, free 125I-anti ACTH (24-39) and bound 125I-anti ACTH (24-39) antibody fractions are separated by discarding the supernatant. Simple wash steps reduce the non-specific binding (NSB) to a minimum for increased precision at the low end of the calibration curve. The concentration of ACTH is directly proportional to the radioactivity bound to the tubes after separation. The concentration of ACTH in unknown samples and controls is determined by interpolation using a calibration curve.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE11081

ACTH Radioimmunoassay
List Price $695
Kit size 100
Method IRMA (CT)
Incubation time 18 - 22 h
Standard range 9.5 - 1800 pg/mL
Specimen / Volumes 200 µL plasma
Substrate / isotope 125I < 740 kBq
Regulatory Status: For research use only