Reverse T3 (rT3) ELISA

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Details for:  Reverse T3 (rT3) ELISA
For the direct quantitative determination of Reverse Triiodothyronine (rT3) in human serum and plasma by an enzyme immunoassay. For research use only. Not for use in diagnostic procedures.
3,3’,5’-Triiodo-L-thyronine also known as reverse triiodothyronine or reverse T3 (rT3), differs from 3,3’,5’-Triiodo-L-thyronine (T3) in the positions of the iodine atoms in the molecule. The majority of circulatory rT3 is synthesized by peripheral deiodination of thyroxine (T4). Both T3 and rT3 bind to thyroid hormone receptors, but in contrast to T3, rT3 has not been found yet to stimulate receptor metabolic activity; it blocks receptor sites from T3 activation. The ratio of rT3 to T3 is a valuable biomarker of the metabolism and function of thyroid hormones because the process of 5’ monodeiodination that converts T4 to T3 and rT3 to 3,3’-T2 is inhibited in a number of non-thyroidal conditions such as fasting, anorexia nervosa, malnutrition, diabetes mellitus, stress, severe trauma or infection, hemorrhagic shock, hepatic dysfunction and others.
This scenario is known as "Sick euthyroid" syndrome or “Low T3” syndrome.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. 30123153

Reverse T3 (rT3) ELISA
List Price $343
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1h (37°C), 1 x 30 min (37°C), 1 x 15 min (37°C)
Standard range 0.02 – 2 ng/mL
Specimen / Volumes 25 µL Serum, EDTA Plasma
Substrate / isotope TMB, 450 nm
Regulatory Status: For research use only


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