T4 Radioimmunoassay

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Details for:  T4 Radioimmunoassay

Radioimmunoassay for the quantitative measurement of human Thyroxine (T4) in serum. For research use only. Not for use in clinical diagnostic procedures.

Test principle

A fixed amount of 125 I labelled T4 competes with the T4 to be measured present in the sample or in the calibrator for a fixed amount of anti-T4 antibody sites, which are bound to the goat anti mouse (GAM) antibodies immobilized to the wall of a polystyrene tube. After 1 hour incubation at room temperature, an aspiration step terminates the competition reaction. The tubes are then washed with 2 ml of working wash solution and aspirated again. A calibration curve is plotted and the T4 concentrations of the samples are determined by dose interpolation from the calibration curve.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. MG13091

T4 Radioimmunoassay
List Price $92
Kit size 96
Method RIA (CT)
Incubation time 1 x 1 h with shaking
Standard range 12.8 - 500 nmol/L
Specimen / Volumes 20 µL Serum, Plasma
Substrate / isotope 125I < 111 kBq
Regulatory Status: For research use only