Thyroid Stimulating Hormone (TSH) ELISA

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Details for:  Thyroid Stimulating Hormone (TSH) ELISA

The TSH ELISA is an enzyme immunoassay for the quantitative measurement of TSH in serum or heparin plasma. For research use only. Not for use in diagnostic procedures.
Not intended for newborn screening.

Test principle

The TSH ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the TSH molecule. An aliquot of sample containing endogenous TSH is incubated in the coated well with enzyme conjugate, which is an anti-TSH antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of TSH in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of TSH in the sample.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE55221

Thyroid Stimulating Hormone (TSH) ELISA
List Price $291
Kit size 12 x 8
Method ELISA
Incubation time 10min, 90 min, 20 min
Standard range 0,25 - 15 mIU/L
Specimen / Volumes 25 µL Serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only