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Details for:  CA15-3 ELISA

Enzyme immunoassay for the quantitative determination of CA 15-3 in human serum and plasma. For research use only. Not for use in diagnostic procedures.

Test principle

The CA 15-3 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the CA15-3 molecule. An aliquot of sample containing endogenous CA15-3 is incubated in the coated well with enzyme conjugate, which is an anti-CA15-3 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA15-3 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA15-3 in the sample.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE54141

List Price $318
Kit size 12 x 8
Method ELISA
Incubation time 1 x 2 h, 1 x 30 min
Standard range 25 - 200 U/mL
Specimen / Volumes 25 µL serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only