Calcitonin ELISA

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Details for:  Calcitonin ELISA

Specific quantitative assay for the determination of calcitonin in human serum. For research use only. Not for use in diagnostic procedures.

Test principle

The Calcitonin Immunoassay is a two-site ELISA [Enzyme-Linked ImmunoSorbent Assay] for the measurement of the biologically intact 32 amino acid chain of Calcitonin. It utilizes two different mouse monoclonal antibodies to human calcitonin specific for well-defined regions on the calcitonin molecule. One antibody binds only to Calcitonin 11-23 and this antibody is biotinylated. The other antibody binds only to Calcitonin 21-32 and this antibody is labeled with horseradish peroxidase [HRP] for detection. In this assay, calibrators, controls, or samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidin-coated microplate well. Thus the calcitonin in the sample is “sandwiched” between these two antibodies. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of calcitonin in the sample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of calcitonin present in the controls and samples are determined directly from this curve.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. NM59011

Calcitonin ELISA
List Price $373
Kit size 12 x 8
Method ELISA
Incubation time 1 x 4h, 1 x 30min
Standard range 10-1000 pg/mL
Specimen / Volumes 100 µL serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only