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Details for:  CYFRA 21-1 ELISA

Enzyme immunoassay for the quantitative determination of CYFRA 21-1 in human serum and plasma. For research use only. Not for use in diagnostic procedures.

Test principle

The IBL CYFRA 21-1 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the CYFRA 21-1 molecule. An aliquot of sample containing endogenous CYFRA 21-1 is incubated in the coated well with enzyme conjugate, which is an anti- CYFRA 21-1 monoclonal antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CYFRA 21-1 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CYFRA 21-1 in the sample.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE54101

List Price $672
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1 h, 1 x 15 min
Standard range 0 - 50 ng/mL
Specimen / Volumes 50 µL serum, plasma
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only