Bordetella PT IgA ELISA

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Details for:  Bordetella PT IgA ELISA
Enzyme immunoassay for the quantitative determination of IgA class antibodies against Bordetella pertussis in human serum or plasma (citrate).The quantitative immunoenzymatic determination of IgA-class antibodies against B. pertussis toxin (PT) is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are coated with B. pertussis toxin (PT) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgA conjugate is added. This conjugate binds to the captured B. pertussis toxin (PT)-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of B. pertussis toxin (PT)-specific IgA antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. 30113475

Bordetella PT IgA ELISA
List Price $171
Kit size 12x8
Method ELISA
Incubation time 1 x 1 h, 1 x 30 min, 1 x 15 min
Standard range 0 - 50 IU / mL
Specimen / Volumes 10 µL serum, plasma
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only