Bordetella pertussis IgA ELISA

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Details for:  Bordetella pertussis IgA ELISA
Enzyme immunoassay for the qualitative determination of IgA class antibodies against Bordetella pertussis. Bordetella species are non-spore-forming encapsulated bipolar, coccoid (pale-staining) Gram-negative bacilli (about 0.3-0.5 μm thick and 1 μm long).

The qualitative immunoenzymatic determination of IgA-class antibodies against Bordetella pertussis is based on the ELISA (Enzyme-linked Immunoorbent Assay) technique. Microtiter strip wells are precoated with Bordetella pertussis/toxin antigens to bind corresponding antibodies
of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgA conjugate is added. This conjugate binds to the captured Bordetella pertussis/toxin specific antibodies. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Bordetella pertussis/toxin specific IgA antibodies in the specimen. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450nm is read using an ELISA microwell plate reader.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. 30113472

Bordetella pertussis IgA ELISA
List Price $171
Kit size 12x8
Method ELISA
Incubation time 1 x 1 h, 1 x 30 min, 1 x 15 min
Standard range cut-off index
Specimen / Volumes 10 µL serum, plasma
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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