Hantavirus (Hantaan) IgG/IgM ELISA

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Details for:  Hantavirus (Hantaan) IgG/IgM ELISA
Enzyme immunoassay for the qualitative determination of IgG/IgM antibodies against the serotype Hantaan of Hantavirus in human serum.
For research use only. Not for use in diagnostic procedures.

TEST PRINCIPLE
The microtiter plate is coated with recombinant nucleo-capsidprotein of Hantaan virus. For determination of IgM antibodies, sera must be incubated with rheumatic-factor-IgG-absorbent before starting the test procedure in order to eliminate unspecific reactions caused by IgG antibodies or rheumatic factor. During the incubation period specific antibodies against the recombinant Hantaan antigen are bound to the solid phase. After washing, the specific IgG and IgM antibodies are detected with peroxidase-conjugated anti human IgG- and IgM antibodies respectively. Addition of substrate solution results in a color reaction, which is proportional to the bound specific antibody content. The absorbance is then measured photometrical.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE57451

Hantavirus (Hantaan) IgG/IgM ELISA
List Price $291
Kit size 12 x 8
Method ELISA
Incubation time 2 x 45 min, 1 x 10 min
Standard range Cut off index
Specimen / Volumes 10 µL serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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