Legionella pneumophila IgG ELISA

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Details for:  Legionella pneumophila IgG ELISA

The Legionella pneumophila IgG-ELISA is intended for the qualitative determination of IgG class antibodies against Legionella pneumophila in human serum or plasma (citrate). For research use only. Not for use in diagnostic procedures.

Test principle

The qualitative immunoenzymatic determination of IgG-class antibodies against Legionella pneumophila is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are coated with Legionella pneumophila antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured Legionella-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Legionella-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader. The qualitative immunoenzymatic determination of IgG class antibodies to Legionella pneumophila is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE58501

Legionella pneumophila IgG ELISA
List Price $217
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1 h, 1 x 30 min, 1 x 15 min
Standard range cut-off index
Specimen / Volumes 10µL serum, plasma
Substrate / isotope TMB 450nm
Regulatory Status: For research use only

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