Leptospira IgM ELISA

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Details for:  Leptospira IgM ELISA

The Leptospira IgM-ELISA is intended for the qualitative determination of IgM class antibodies against Leptospira spp. in human serum or plasma (heparin). For research use only. Not for use in diagnostic procedures.

Test principle

The qualitative immunoenzymatic determination of IgM-class antibodies against Leptospira spp. is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are coated with Leptospira antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled antihuman IgM conjugate is added. This conjugate binds to the captured Leptospira-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB)substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Leptospira-specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE58941

Leptospira IgM ELISA
List Price $175
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1h, 1 x 30min, 1 x 15min
Standard range cut-off index
Specimen / Volumes 10µL serum, plasma
Substrate / isotope TMB 450nm
Regulatory Status: For research use only