Measles virus IgM µ-capture ELISA

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Details for:  Measles virus IgM µ-capture ELISA
Enzyme immunoassay for the quantitative and quantitative determination of IgM antibodies to measles virus in human serum and plasma. For research use only. Not for use in diagnostic procedures.

TEST PRINCIPLE
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the µ-capture principle. Anti-human-IgM-antibodies are bound on the surface of the microtiter strips. Diluted serum or ready to use calibrators and controls are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the sample and the IgM class-specific antibodies on the microtiter plate takes place during the first incubation.
In the second incubation, ready to use antigen conjugate (measles antigen conjugated to peroxidise) is added and binds to specific IgM antibodies captured on the microtiter wells.
Subsequently, substrate (TMB) is pipetted, inducing the development of a blue dye in the wells. The colour development is terminated by the addition of a stop solution, which courses the colour change from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the specific IgM antibodies is directly proportional to the intensity of the colour.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE57151

Measles virus IgM µ-capture ELISA
List Price $178
Kit size 12 x 8
Method ELISA
Incubation time 2 x 1 h, 1 x 30 min
Standard range 2-200 U/ml
Specimen / Volumes 10 µl serum, plasma
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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