Neopterin ELISA

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Details for:  Neopterin ELISA

Enzyme immunoassay for the quantitative determination of neopterin in human serum, plasma and urine. For research use only. Not for use in clinical diagnostic procedures.

Solid phase enzyme-linked immunosorbent assay (ELISA) based on the basic principle of a competitive ELISA. An unknown amount of antigen in the sample and a fixed amount of enzyme labelled antigen compete for the antibody-binding sites (rabbit-anti-neopterin). Both antigen-antibody complexes bind to the wells of the microtiter strips coated with a goat-anti-rabbit antibody. Unbound antigen is removed by washing. The intensity of the color developed after the substrate incubation is inversely proportional to the amount of antigen in the sample. Results of samples can be determined directly using the standard curve.

Neopterin is a low molecular weight molecule belonging to the chemical group known as pteridines. It is synthesised by cellular immune reaction of macrophages and dendritic cells upon stimulation with the cytokine interferon-g and as a consequence released. Neopterin has a higher stability in body fluids which makes the sample handling and measurement easier compared to other cytokines. Due to its low molecular weight, neopterin can rapidly pass the intravasal area, where it is releases in urine after glomerular filtration. The half life period in human bodies is only affected by renal excretion.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE59321

Neopterin ELISA
List Price $404
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1.5 h, 1 x 10 min
Standard range 1.35 - 111 nmol/L
Specimen / Volumes 20 µL serum, plasma, urine
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only


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