Malaria-Ab ELISA

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Details for:  Malaria-Ab ELISA
The Malaria-Ab ELISA is intended for the qualitative determination of antibodies against Plasmodium in human serum or plasma (citrate). For research use only. Not for use in diagnostic procedures.

PRINCIPLE OF THE ASSAY
The qualitative immunoenzymatic determination of antibodies against Plasmodium is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Plasmodium antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG and IgM conjugate is added. This conjugate binds to the captured Plasmodiumspecific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Plasmodium-specific antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE58901

Malaria-Ab ELISA
List Price $163
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1 h, 1 x 30 min, 1 x 15 min
Standard range Cutoff index
Specimen / Volumes 100 µL serum and plasma
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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