Dengue virus IgM ELISA

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Details for:  Dengue virus IgM ELISA
The Dengue virus IgM ELISA is intended for the qualitative determination of IgM class antibodies against Dengue virus in human serum or plasma (citrate). For research use only. Not for use indiagnostic procedures.

PRINCIPLE OF THE ASSAY
The qualitative immunoenzymatic determination of IgM-class antibodies against Dengue Virus is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiter strip wells are precoated with Dengue Virus antigens type 2 to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Dengue Virusspecific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Dengue Virus-specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE58681

Dengue virus IgM ELISA
List Price $163
Kit size 12 x 8
Method ELISA
Incubation time 1 x 1h, 1 x 30min, 1 x 15min
Standard range cut-off index
Specimen / Volumes 10 µL serum
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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