LH Urine ELISA

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Details for:  LH Urine ELISA
The LH Urine ELISA is an enzyme immunoassay for the quantitative measurement of LH in urine.
For research use only. Not for use in diagnostic procedures.

SUMMARY AND EXPLANATION
Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), which is released by the hypothalamus.
LH, also called interstitial cell-stimulating hormone (ICSH) in men, is a glycoprotein with a molecular weight of approximately 30.000 daltons. It is composed of two non covalently associated dissimilar amino acid chains, alpha and beta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH), follicle stimulating hormone (FSH), and human chorionic gonadotropin (hCG). The difference between these hormones lie in the amino acid composition of their beta subunits, which account for their immunological differentiation..

PRINCIPLE OF THE TEST
The LH Urine ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site of the β-LH molecule. An aliquot of sample containing endogenous LH is incubated in the coated well with enzyme conjugate, which is an anti- LH antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off.
The amount of bound peroxidase is proportional to the concentration of LH in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of LH in the sample.
For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. RE52111

LH Urine ELISA
List Price $217
Kit size 12 x 8
Method ELISA
Incubation time 1 x 30 min, 1 x 10 min
Standard range 10 - 200 mIU/mL
Specimen / Volumes 25 µL Urine
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only

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