Testosterone Saliva ELISA

Improved Saliva Testing of hormone levels

Enzyme immunoassay for the quantitative determination of free testosterone in human saliva.

Benefits of the Testosterone Saliva ELISA

• Calibration to the LC-MS/MS
• Simplified pipetting protocol
• Alignment to entire Saliva line

Consistent and reliable processing within Saliva product line

Our Saliva ELISA product line (Cortisol, Testosterone, Progesterone, DHEA) can be processed in a similar way and be easily performed in combination:

• The TMB, washing and stop solutions are interchangeable;
• The enzyme conjugate, the substrate and stop solutions are uniformly pipetted with 100 µL;
• The kits contain as few as 6 standards and 2 controls;
• Only 50 µL sample volume are required;
• All kits use the same sample diluent buffer.

Automation

The IBL International Testosterone Saliva ELISA (RE52631) can be adapted on different ELISA processors. The combined use of assays, process script and automate has to be validated individually on site by each laboratory.

Measurement of Testosterone in saliva

Only 1-2% of the hormone Testosterone is unbound from plasma proteins and therefore biologically active. It is released via the salivary glands and mainly transformed to 5a-dihydrotestosterone. The concentration of testosterone in saliva, reflects however the level of free testosterone in plasma.

Pulsatile dynamics

Due to the pulsatile dynamics of Testosterone secretion repeated saliva sampling is recommended. We recommend collecting 3 to 5 saliva samples within 2 hours. In the laboratory, equal volumes of the individual saliva samples can be mixed. This mixed sample results in a mean free Testosterone value, which represents the active hormone concentration in a reproducible way.

Therefore measuring the free testosterone in saliva is a convenient non-invasive method. Easier sample collection without repeated venipunctures and reduced stress on the specimen offer benefits to the patient while sensibility and accuracy of the results are ensured.