TNF-alpha (TNF-a) ELISA

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Details for:  TNF-alpha (TNF-a) ELISA
Formerly sold as BE55001.

The TNF alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TNF alpha in cell culture supernatant, serum ans plasma (EDTA, citrate and heparin). For research use only. Not for use in diagnostic procedures.

Principle of test:

An anti-human TNF alpha coating antibody is adsorbed onto microwells. Human TNF alpha present in the sample or standard binds to antibodies adsorbed to the microwells. A biotin-conjugated anti-human TNF alpha antibody is added and binds to human TNF alpha captured by the first antibody. Following incubation unbound biotinconjugated anti-human TNF alpha antibody is removed during a wash step. Streptavidin- HRP is added and binds to the biotinconjugated anti-human TNF alpha antibody. Following incubation unbound Streptavidin- HRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human TNF alpha present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human TNF alpha standard dilutions and human TNF alpha sample concentration determined.

For concrete data please consult the Instruction for Use in the download box on the right side.

Catalog No. 30147293

TNF-alpha (TNF-a) ELISA
List Price $501
Kit size 12 x 8
Method ELISA
Incubation time 1 x 2 h, 1 x 1 h, 1 x 10 min
Standard range 23 - 1500 pg/mL
Specimen / Volumes 100 µL serum, plasma, urine, cell culture supernatant, saliva et al.
Substrate / isotope TMB 450 nm
Regulatory Status: For research use only